Nature of the fructan of Streptococcus mutans OMZ 176.

Isolation and purification of Flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of Streptococcus mutans.

Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by S. mutans OMZ ...

Growth of several cariogenic strains of oral streptococci in a chemically defined medium.

A chemically defined medium in which Streptococcus mutans strains AHT, BHT, GS-5, JC-2, Ingbritt, At6T, At9T, 6715, and OMZ-176 and Streptococcus salivarius strain HHT grew rapidly to high turbidities was formulated. Maximal turbidities of each strain were observed after 8 to 12 h of aerobic growth. The subsequent transfer of exponentially growing cells into fresh medium resulted in growth at t...

Effects of Streptococcus mutans gtfC deficiency on mixed oral biofilms in vitro.

The aim of this study was to examine the influence of glucosyltransferase-gene-negative (gtf-) Streptococcus mutans strains unable to synthesize water-insoluble or soluble glucan on the structure and macromolecular diffusion properties of in vitro grown mixed oral biofilms. Biofilms modeling supragingival plaque consisted of Actinomyces naeslundii OMZ 745, Candida albicans OMZ 110, Fusobacteriu...

Use of specifically labeled sucrose for comparison of extracellular glucan and fructan metabolism by oral streptococci.

Sucrose labeled in the fructosyl ((3)H) and glucosyl ((14)C) moieties was used to quantitate extracellular polysaccharide production and degradation by cariogenic and noncariogenic oral streptococci. All of the strains produced glucan and fructan. Streptococcus salivarius produced primarily fructan, whereas S. mutans and S. sanguis produced more glucan than fructan. The cariogenic streptococci ...

The collagen binding protein Cnm contributes to oral 1 colonization and cariogenicity of Streptococcus mutans 2 OMZ 175 . 3 4